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Cellosaurus NUGC-3 (CVCL_1612)

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Cell line name NUGC-3
Synonyms NUGC3; NU-GC-3; Nagoya University-Gastric Cancer-3
Accession CVCL_1612
Secondary accession CVCL_8371
Resource Identification Initiative To cite this cell line use: NUGC-3 (RRID:CVCL_1612)
Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: COSMIC cell lines project.
Part of: JFCR45 cancer cell line panel.
Part of: MD Anderson Cell Lines Project.
Population: Japanese.
Doubling time: 38.2 hours (PubMed=3172586); 29 hours (PubMed=29435981); ~26 hours (Note=Lot 06062005), 28-45 hours (Note=Lot 04272009), ~32 hours (Note=Lot 11042011), ~28 hours (Note=Lot 02262016), ~1 day (Note=Lot 11162017) (JCRB=JCRB0822).
Microsatellite instability: Instable (MSI-high) (PubMed=31068700; Sanger).
Omics: Array-based CGH.
Omics: CRISPR phenotypic screen.
Omics: Deep exome analysis.
Omics: Deep quantitative proteome analysis.
Omics: DNA methylation analysis.
Omics: Protein expression by reverse-phase protein arrays.
Omics: SNP array analysis.
Omics: Transcriptome analysis by microarray.
Omics: Transcriptome analysis by RNAseq.
Misspelling: MUGC-3; Cosmic=1187288; PubMed=19132987.
Misspelling: NVGC3; Cosmic=801341.
Derived from site: Metastatic; Brachialis muscle; UBERON=UBERON_0001506.
Sequence variations
  • Mutation; HGNC; 11998; TP53; Simple; p.Tyr220Cys (c.659A>G); ClinVar=VCV000127819; Zygosity=Unspecified (PubMed=1370612).
HLA typing Source: PubMed=26589293
Class I
HLA-AA*24:02,24:02
HLA-BB*52:01,52:01
HLA-CC*12:02,12:02
Class II
HLA-DQDQA1*05:02,05:02
DQB1*06:01,06:01
HLA-DRDRB1*15:02,15:02
Genome ancestry Source: PubMed=30894373

Origin% genome
African0
Native American0.09
East Asian, North82.25
East Asian, South16.95
South Asian0
European, North0
European, South0.71
Disease Gastric adenocarcinoma (NCIt: C4004)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_4Y07 (NUGC-3-Luc)CVCL_L502 (NUGC-3/5-FU/L)
Sex of cell Male
Age at sampling 72Y
Category Cancer cell line
STR profile Source(s): Cosmic-CLP=908455; JCRB=JCRB0822; PubMed=25877200

Markers:
AmelogeninX
CSF1PO13
D3S135816,17
D5S81811
D7S8208,9
D8S117912,13,16,17
D13S31710,11
D16S53910,11
D18S5118,19
D21S1130,31
FGA17
Penta D11
Penta E11,18
TH016,8
TPOX8,9
vWA18

Run an STR similarity search on this cell line
Web pages https://tcpaportal.org/mclp/
Publications

PubMed=3325673; DOI=10.1007/BF02470755
Watanabe T., Nishikimi M., Akiyama S., Sakamoto J., Shiku H., Ichihashi H., Ozawa T., Kondo T., Takagi H.
A stomach oncofetal antigen recognized by monoclonal antibody GC302.
Jpn. J. Surg. 17:507-516(1987)

PubMed=3172586; DOI=10.1007/BF02471470
Akiyama S., Amo H., Watanabe T., Matsuyama M., Sakamoto J., Imaizumi M., Ichihashi H., Kondo T., Takagi H.
Characteristics of three human gastric cancer cell lines, NU-GC-2, NU-GC-3 and NU-GC-4.
Jpn. J. Surg. 18:438-446(1988)

PubMed=1778766; DOI=10.1111/j.1349-7006.1991.tb01816.x; PMCID=PMC5918361
Takeshima E., Hamaguchi M., Watanabe T., Akiyama S., Kataoka M., Ohnishi Y., Xiao H.-Y., Nagai Y., Takagi H.
Aberrant elevation of tyrosine-specific phosphorylation in human gastric cancer cells.
Jpn. J. Cancer Res. 82:1428-1435(1991)

PubMed=1370612; DOI=10.1016/S0006-291X(05)80133-0
Matozaki T., Sakamoto C., Matsuda K., Suzuki T., Konda Y., Nakano O., Wada K., Uchida T., Nishisaki H., Nagao M., Kasuga M.
Missense mutations and a deletion of the p53 gene in human gastric cancer.
Biochem. Biophys. Res. Commun. 182:215-223(1992)

PubMed=9290701; DOI=10.1002/(SICI)1098-2744(199708)19:4<243::AID-MC5>3.0.CO;2-D
Jia L.-Q., Osada M., Ishioka C., Gamo M., Ikawa S., Suzuki T., Shimodaira H., Niitani T., Kudo T., Akiyama M., Kimura N., Matsuo M., Mizusawa H., Tanaka N., Koyama H., Namba M., Kanamaru R., Kuroki T.
Screening the p53 status of human cell lines using a yeast functional assay.
Mol. Carcinog. 19:243-253(1997)

PubMed=15723654; DOI=10.1111/j.1349-7006.2005.00016.x; PMCID=PMC11160020
Takada H., Imoto I., Tsuda H., Sonoda I., Ichikura T., Mochizuki H., Okanoue T., Inazawa J.
Screening of DNA copy-number aberrations in gastric cancer cell lines by array-based comparative genomic hybridization.
Cancer Sci. 96:100-110(2005)

PubMed=15767549; DOI=10.1158/1535-7163.MCT-04-0234
Nakatsu N., Yoshida Y., Yamazaki K., Nakamura T., Dan S., Fukui Y., Yamori T.
Chemosensitivity profile of cancer cell lines and identification of genes determining chemosensitivity by an integrated bioinformatical approach using cDNA arrays.
Mol. Cancer Ther. 4:399-412(2005)

PubMed=19132987; DOI=10.1111/j.1600-0463.2008.01042.x
Li Y.-Q., Tanaka Y., Tada M., Hua R., Seto M., Asaoka Y., Ohta M., Kanai F., Yoshida H., Kawabe T., Sano T., Motojima T., Yokosuka O., Omata M.
Absence of the AKT1 pleckstrin homology domain mutation in Japanese gastrointestinal and liver cancer patients.
APMIS 116:931-933(2008)

PubMed=20164919; DOI=10.1038/nature08768; PMCID=PMC3145113
Bignell G.R., Greenman C.D., Davies H.R., Butler A.P., Edkins S., Andrews J.M., Buck G., Chen L., Beare D., Latimer C., Widaa S., Hinton J., Fahey C., Fu B.-Y., Swamy S., Dalgliesh G.L., Teh B.T., Deloukas P., Yang F.-T., Campbell P.J., Futreal P.A., Stratton M.R.
Signatures of mutation and selection in the cancer genome.
Nature 463:893-898(2010)

PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458; PMCID=PMC2881662
Rothenberg S.M., Mohapatra G., Rivera M.N., Winokur D., Greninger P., Nitta M., Sadow P.M., Sooriyakumar G., Brannigan B.W., Ulman M.J., Perera R.M., Wang R., Tam A., Ma X.-J., Erlander M., Sgroi D.C., Rocco J.W., Lingen M.W., Cohen E.E.W., Louis D.N., Settleman J., Haber D.A.
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
Cancer Res. 70:2158-2164(2010)

PubMed=22460905; DOI=10.1038/nature11003; PMCID=PMC3320027
Barretina J.G., Caponigro G., Stransky N., Venkatesan K., Margolin A.A., Kim S., Wilson C.J., Lehar J., Kryukov G.V., Sonkin D., Reddy A., Liu M., Murray L., Berger M.F., Monahan J.E., Morais P., Meltzer J., Korejwa A., Jane-Valbuena J., Mapa F.A., Thibault J., Bric-Furlong E., Raman P., Shipway A., Engels I.H., Cheng J., Yu G.-Y.K., Yu J.-J., Aspesi P. Jr., de Silva M., Jagtap K., Jones M.D., Wang L., Hatton C., Palescandolo E., Gupta S., Mahan S., Sougnez C., Onofrio R.C., Liefeld T., MacConaill L.E., Winckler W., Reich M., Li N.-X., Mesirov J.P., Gabriel S.B., Getz G., Ardlie K., Chan V., Myer V.E., Weber B.L., Porter J., Warmuth M., Finan P., Harris J.L., Meyerson M.L., Golub T.R., Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Nature 483:603-607(2012)

PubMed=24807215; DOI=10.1038/ncomms4830; PMCID=PMC4024760
Liu J.-F., McCleland M.L., Stawiski E.W., Gnad F., Mayba O., Haverty P.M., Durinck S., Chen Y.-J., Klijn C., Jhunjhunwala S., Lawrence M., Liu H.-B., Wan Y.-N., Chopra V.S., Yaylaoglu M.B., Yuan W.-L., Ha C., Gilbert H.N., Reeder J., Pau G., Stinson J., Stern H.M., Manning G., Wu T.D., Neve R.M., de Sauvage F.J., Modrusan Z., Seshagiri S., Firestein R., Zhang Z.-M.
Integrated exome and transcriptome sequencing reveals ZAK isoform usage in gastric cancer.
Nat. Commun. 5:3830.1-3830.8(2014)

PubMed=25343454; DOI=10.1371/journal.pone.0111146; PMCID=PMC4208810
Choong M.-L., Tan S.-H., Tan T.-Z., Manesh S., Ngo A., Yong J.W.-Y., Yang H.H., Lee M.A.
Molecular integrative clustering of Asian gastric cell lines revealed two distinct chemosensitivity clusters.
PLoS ONE 9:E111146-E111146(2014)

PubMed=25485619; DOI=10.1038/nbt.3080
Klijn C., Durinck S., Stawiski E.W., Haverty P.M., Jiang Z.-S., Liu H.-B., Degenhardt J., Mayba O., Gnad F., Liu J.-F., Pau G., Reeder J., Cao Y., Mukhyala K., Selvaraj S.K., Yu M.-M., Zynda G.J., Brauer M.J., Wu T.D., Gentleman R.C., Manning G., Yauch R.L., Bourgon R., Stokoe D., Modrusan Z., Neve R.M., de Sauvage F.J., Settleman J., Seshagiri S., Zhang Z.-M.
A comprehensive transcriptional portrait of human cancer cell lines.
Nat. Biotechnol. 33:306-312(2015)

PubMed=25877200; DOI=10.1038/nature14397
Yu M., Selvaraj S.K., Liang-Chu M.M.Y., Aghajani S., Busse M., Yuan J., Lee G., Peale F.V., Klijn C., Bourgon R., Kaminker J.S., Neve R.M.
A resource for cell line authentication, annotation and quality control.
Nature 520:307-311(2015)

PubMed=26589293; DOI=10.1186/s13073-015-0240-5; PMCID=PMC4653878
Scholtalbers J., Boegel S., Bukur T., Byl M., Goerges S., Sorn P., Loewer M., Sahin U., Castle J.C.
TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.
Genome Med. 7:118.1-118.7(2015)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017; PMCID=PMC4967469
Iorio F., Knijnenburg T.A., Vis D.J., Bignell G.R., Menden M.P., Schubert M., Aben N., Goncalves E., Barthorpe S., Lightfoot H., Cokelaer T., Greninger P., van Dyk E., Chang H., de Silva H., Heyn H., Deng X.-M., Egan R.K., Liu Q.-S., Mironenko T., Mitropoulos X., Richardson L., Wang J.-H., Zhang T.-H., Moran S., Sayols S., Soleimani M., Tamborero D., Lopez-Bigas N., Ross-Macdonald P., Esteller M., Gray N.S., Haber D.A., Stratton M.R., Benes C.H., Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

PubMed=28196595; DOI=10.1016/j.ccell.2017.01.005; PMCID=PMC5501076
Li J., Zhao W., Akbani R., Liu W.-B., Ju Z.-L., Ling S.-Y., Vellano C.P., Roebuck P., Yu Q.-H., Eterovic A.K., Byers L.A., Davies M.A., Deng W.-L., Gopal Y.N.V., Chen G., von Euw E.M., Slamon D.J., Conklin D., Heymach J.V., Gazdar A.F., Minna J.D., Myers J.N., Lu Y.-L., Mills G.B., Liang H.
Characterization of human cancer cell lines by reverse-phase protein arrays.
Cancer Cell 31:225-239(2017)

PubMed=29435981; DOI=10.1002/ijc.31304
Kim H.J., Kang S.K., Kwon W.S., Kim T.S., Jeong I., Jeung H.-C., Kragh M., Horak I.D., Chung H.C., Rha S.Y.
Forty-nine gastric cancer cell lines with integrative genomic profiling for development of c-MET inhibitor.
Int. J. Cancer 143:151-159(2018)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747; PMCID=PMC6445675
Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=30971826; DOI=10.1038/s41586-019-1103-9
Behan F.M., Iorio F., Picco G., Goncalves E., Beaver C.M., Migliardi G., Santos R., Rao Y., Sassi F., Pinnelli M., Ansari R., Harper S., Jackson D.A., McRae R., Pooley R., Wilkinson P., van der Meer D.J., Dow D., Buser-Doepner C.A., Bertotti A., Trusolino L., Stronach E.A., Saez-Rodriguez J., Yusa K., Garnett M.J.
Prioritization of cancer therapeutic targets using CRISPR-Cas9 screens.
Nature 568:511-516(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3; PMCID=PMC6697103
Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C., McDonald E.R. 3rd, Barretina J.G., Gelfand E.T., Bielski C.M., Li H.-X., Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F., Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R., Lu Y.-L., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C., Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A., Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D., Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L., Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A., Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D., Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M., Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R., Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A., Sellers W.R.
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)

PubMed=31978347; DOI=10.1016/j.cell.2019.12.023; PMCID=PMC7339254
Nusinow D.P., Szpyt J., Ghandi M., Rose C.M., McDonald E.R. 3rd, Kalocsay M., Jane-Valbuena J., Gelfand E.T., Schweppe D.K., Jedrychowski M.P., Golji J., Porter D.A., Rejtar T., Wang Y.K., Kryukov G.V., Stegmeier F., Erickson B.K., Garraway L.A., Sellers W.R., Gygi S.P.
Quantitative proteomics of the Cancer Cell Line Encyclopedia.
Cell 180:387-402.e16(2020)

PubMed=35839778; DOI=10.1016/j.ccell.2022.06.010; PMCID=PMC9387775
Goncalves E., Poulos R.C., Cai Z.-X., Barthorpe S., Manda S.S., Lucas N., Beck A., Bucio-Noble D., Dausmann M., Hall C., Hecker M., Koh J., Lightfoot H., Mahboob S., Mali I., Morris J., Richardson L., Seneviratne A.J., Shepherd R., Sykes E., Thomas F., Valentini S., Williams S.G., Wu Y.-X., Xavier D., MacKenzie K.L., Hains P.G., Tully B., Robinson P.J., Zhong Q., Garnett M.J., Reddel R.R.
Pan-cancer proteomic map of 949 human cell lines.
Cancer Cell 40:835-849.e8(2022)

Cross-references
Cell line collections (Providers) JCRB; JCRB0822
Cell line databases/resources CLO; CLO_0037144
cancercelllines; CVCL_1612
Cell_Model_Passport; SIDM00572
CGH-DB; 134-1
Cosmic-CLP; 908455
DepMap; ACH-000911
LINCS_LDP; LCL-1887
Anatomy/cell type resources BTO; BTO:0002597
Biological sample resources BioSample; SAMN03473123
BioSample; SAMN10988359
CRISP screens repositories BioGRID_ORCS_Cell_line; 397
Chemistry resources ChEMBL-Cells; CHEMBL3307562
ChEMBL-Targets; CHEMBL614208
GDSC; 908455
PharmacoDB; NUGC3_1173_2019
PubChem_Cell_line; CVCL_1612
Encyclopedic resources Wikidata; Q54931160
Experimental variables resources EFO; EFO_0006702
Gene expression databases ArrayExpress; E-MTAB-783
ArrayExpress; E-MTAB-2706
ArrayExpress; E-MTAB-2770
ArrayExpress; E-MTAB-3610
GEO; GSM552367
GEO; GSM827405
GEO; GSM828828
GEO; GSM844666
GEO; GSM887462
GEO; GSM888542
GEO; GSM1374776
GEO; GSM1670287
Polymorphism and mutation databases Cosmic; 801341
Cosmic; 877046
Cosmic; 908455
Cosmic; 918499
Cosmic; 968351
Cosmic; 1187288
Cosmic; 1518256
Cosmic; 1995599
Cosmic; 2484968
Cosmic; 2807638
IARC_TP53; 973
LiGeA; CCLE_449
Progenetix; CVCL_1612
Proteomic databases PRIDE; PXD030304
Sequence databases EGA; EGAS00001000610
EGA; EGAS00001000978
Entry history
Entry creation04-Apr-2012
Last entry update10-Sep-2024
Version number44