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Cellosaurus Karpas-45 (CVCL_1326)

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Cell line name Karpas-45
Synonyms KARPAS-45; Karpas 45; KARPAS 45; KARPAS45; K45; T-45; T45; Line 45
Accession CVCL_1326
Resource Identification Initiative To cite this cell line use: Karpas-45 (RRID:CVCL_1326)
Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: COSMIC cell lines project.
Population: Caucasian.
Doubling time: 50-100 hours (ECACC=06072602).
Microsatellite instability: Stable (MSS) (Sanger).
Microsatellite instability: Instable (MSI) (PubMed=31068700).
Omics: Deep exome analysis.
Omics: DNA methylation analysis.
Omics: Deep quantitative proteome analysis.
Omics: SNP array analysis.
Omics: Transcriptome analysis by microarray.
Omics: Transcriptome analysis by RNAseq.
Derived from site: In situ; Bone marrow; UBERON=UBERON_0002371.
Cell type: T-cell; CL=CL_0000084.
Sequence variations
  • Gene fusion; HGNC; 7139; FOXO4 + HGNC; 7132; KMT2A; Name(s)=KMT2A-FOXO4, MLL-MLLT7; MLL-AFX1 (PubMed=14671638; PubMed=15843827).
  • Mutation; HGNC; 583; APC; Simple; p.Val2194Ala (c.6581T>C); ClinVar=VCV000565658; Zygosity=Heterozygous (PubMed=22675565; Cosmic-CLP=907272; DepMap=ACH-002256).
  • Mutation; HGNC; 1787; CDKN2A; Simple; p.Arg80Ter (c.238C>T) (p.Pro94Leu, c.281C>T); ClinVar=VCV000009409; Zygosity=Homozygous (DepMap=ACH-002256).
  • Mutation; HGNC; 3430; ERBB2; Simple; p.Ala586Gly (c.1757C>G); Zygosity=Heterozygous (PubMed=22675565; Cosmic-CLP=907272; DepMap=ACH-002256).
  • Mutation; HGNC; 3495; ETV6; Simple; p.His328Tyr (c.982C>T); Zygosity=Heterozygous (PubMed=22675565; Cosmic-CLP=907272; DepMap=ACH-002256).
  • Mutation; HGNC; 16712; FBXW7; Simple; p.Arg505Cys (c.1513C>T); ClinVar=VCV000069961; Zygosity=Heterozygous (PubMed=22675565; Cosmic-CLP=907272; DepMap=ACH-002256).
  • Mutation; HGNC; 3689; FGFR2; Simple; p.Met584Arg (c.1751T>G) (p.Met585Arg, c.1754T>G); Zygosity=Heterozygous (PubMed=22675565; Cosmic-CLP=907272; DepMap=ACH-002256).
  • Mutation; HGNC; 6307; KDR; Simple; p.Arg962His (c.2885G>A); Zygosity=Heterozygous (PubMed=22675565; Cosmic-CLP=907272; DepMap=ACH-002256).
  • Mutation; HGNC; 6342; KIT; Simple; p.Asp131Asn (c.391G>A); ClinVar=VCV000409719; Zygosity=Heterozygous (PubMed=22675565; Cosmic-CLP=907272; DepMap=ACH-002256).
  • Mutation; HGNC; 7029; MET; Simple; p.Ala48Thr (c.142G>A); ClinVar=VCV000454188; Zygosity=Heterozygous (PubMed=22675565; Cosmic-CLP=907272; DepMap=ACH-002256).
  • Mutation; HGNC; 7029; MET; Simple; p.Glu157Gly (c.470A>G); Zygosity=Heterozygous (PubMed=22675565; Cosmic-CLP=907272; DepMap=ACH-002256).
  • Mutation; HGNC; 7325; MSH2; Simple; p.Gly221Val (c.662G>T); Zygosity=Heterozygous (PubMed=22675565; Cosmic-CLP=907272; DepMap=ACH-002256).
  • Mutation; HGNC; 7881; NOTCH1; Simple; p.Gln2459Ter (c.7375C>T); Zygosity=Heterozygous (PubMed=15472075).
  • Mutation; HGNC; 7881; NOTCH1; Simple; p.Asp1852Asn (c.5554G>A); Zygosity=Heterozygous (PubMed=22675565; Cosmic-CLP=907272; DepMap=ACH-002256).
  • Mutation; HGNC; 7881; NOTCH1; Simple; p.Val2045Ile (c.6133G>A); Zygosity=Heterozygous (PubMed=22675565; Cosmic-CLP=907272; DepMap=ACH-002256).
  • Mutation; HGNC; 8804; PDGFRB; Simple; p.His277Gln (c.831C>A); Zygosity=Heterozygous (PubMed=22675565; Cosmic-CLP=907272; DepMap=ACH-002256).
  • Mutation; HGNC; 9967; RET; Simple; p.Asn879Ser (c.2636A>G); Zygosity=Heterozygous (PubMed=22675565; Cosmic-CLP=907272; DepMap=ACH-002256).
  • Mutation; HGNC; 11103; SMARCB1; Simple; p.Asn288Lys (c.864C>A); Zygosity=Heterozygous (PubMed=22675565; Cosmic-CLP=907272; DepMap=ACH-002256).
  • Mutation; HGNC; 11998; TP53; Simple; p.Arg273Cys (c.817C>T); ClinVar=VCV000043594; Zygosity=Heterozygous (PubMed=22675565; Cosmic-CLP=907272; DepMap=ACH-002256).
  • Mutation; HGNC; 12796; WT1; Simple; p.Arg394Gln (c.1181G>A) (p.Arg467Gln, c.1400G>A); ClinVar=VCV000419332; Zygosity=Heterozygous (PubMed=22675565; Cosmic-CLP=907272).
Genome ancestry Source: PubMed=30894373

Origin% genome
African0.06
Native American0.47
East Asian, North0.03
East Asian, South0
South Asian2.75
European, North68.54
European, South28.16
Disease Childhood T acute lymphoblastic leukemia (NCIt: C7953)
Precursor T-cell acute lymphoblastic leukemia (ORDO: Orphanet_99861)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Sex of cell Male
Age at sampling 2Y
Category Cancer cell line
STR profile Source(s): Cosmic-CLP=907272; DSMZ=ACC-105

Markers:
AmelogeninX,Y
CSF1PO11,14
D2S133817,26
D3S135814,15
D5S81811,12
D7S8208,11
D8S117911,15
D13S3179,14
D16S5399,13
D18S5116,18
D19S43314
D21S1130,31.2
FGA22,23
Penta D12,13
Penta E5,10
TH019
TPOX8
vWA14,18

Run an STR similarity search on this cell line
Publications

DOI=10.1016/0145-2126(77)90063-7
Karpas A., Hayhoe F.G.J., Greenberger J.S., Barker C.R., Cawley J.C., Lowenthal R.M., Moloney W.C.
The establishment and cytological, cytochemical and immunological characterisation of human haemic cell lines: evidence for heterogeneity.
Leuk. Res. 1:35-49(1977)

DOI=10.1007/978-1-4613-2823-0_29
Karpas A.
Viruses and human leukemia in vitro.
(In book chapter) Human leukemia. Cytochemical and ultrastructural techniques in diagnosis and research. Developments in Oncology, Vol. 14; Polliack A. (eds.); pp.495-526; Martinus Nijhoff Publishing; Boston; USA (1984)

PubMed=3264071; DOI=10.1073/pnas.85.23.9229; PMCID=PMC282712
Karpas A., Fleet G.W.J., Dwek R.A., Petursson S., Namgoong S.K., Ramsden N.G., Jacob G.S., Rademacher T.W.
Aminosugar derivatives as potential anti-human immunodeficiency virus agents.
Proc. Natl. Acad. Sci. U.S.A. 85:9229-9233(1988)

PubMed=9933131; DOI=10.1016/S0145-2126(98)00133-7
Burger R., Hansen-Hagge T.E., Drexler H.G., Gramatzki M.
Heterogeneity of T-acute lymphoblastic leukemia (T-ALL) cell lines: suggestion for classification by immunophenotype and T-cell receptor studies.
Leuk. Res. 23:19-27(1999)

DOI=10.1016/B978-0-12-221970-2.50457-5
Drexler H.G.
The leukemia-lymphoma cell line factsbook.
(In book) ISBN 9780122219702; pp.1-733; Academic Press; London; United Kingdom (2001)

PubMed=14671638; DOI=10.1038/sj.leu.2403236
Drexler H.G., Quentmeier H., MacLeod R.A.F.
Malignant hematopoietic cell lines: in vitro models for the study of MLL gene alterations.
Leukemia 18:227-232(2004)

PubMed=15472075; DOI=10.1126/science.1102160
Weng A.P., Ferrando A.A., Lee W., Morris J.P. 4th, Silverman L.B., Sanchez-Irizarry C., Blacklow S.C., Look A.T., Aster J.C.
Activating mutations of NOTCH1 in human T cell acute lymphoblastic leukemia.
Science 306:269-271(2004)

PubMed=15843827; DOI=10.1038/sj.leu.2403749
Andersson A., Eden P., Lindgren D., Nilsson J., Lassen C., Heldrup J., Fontes M., Borg A., Mitelman F., Johansson B., Hoglund M., Fioretos T.
Gene expression profiling of leukemic cell lines reveals conserved molecular signatures among subtypes with specific genetic aberrations.
Leukemia 19:1042-1050(2005)

PubMed=16408098; DOI=10.1038/sj.leu.2404081
Quentmeier H., MacLeod R.A.F., Zaborski M., Drexler H.G.
JAK2 V617F tyrosine kinase mutation in cell lines derived from myeloproliferative disorders.
Leukemia 20:471-476(2006)

PubMed=17170727; DOI=10.1038/sj.leu.2404486
Sandberg Y., Verhaaf B., van Gastel-Mol E.J., Wolvers-Tettero I.L.M., De Vos J., MacLeod R.A.F., Noordzij J.G., Dik W.A., van Dongen J.J.M., Langerak A.W.
Human T-cell lines with well-defined T-cell receptor gene rearrangements as controls for the BIOMED-2 multiplex polymerase chain reaction tubes.
Leukemia 21:230-237(2007)

PubMed=20164919; DOI=10.1038/nature08768; PMCID=PMC3145113
Bignell G.R., Greenman C.D., Davies H.R., Butler A.P., Edkins S., Andrews J.M., Buck G., Chen L., Beare D., Latimer C., Widaa S., Hinton J., Fahey C., Fu B.-Y., Swamy S., Dalgliesh G.L., Teh B.T., Deloukas P., Yang F.-T., Campbell P.J., Futreal P.A., Stratton M.R.
Signatures of mutation and selection in the cancer genome.
Nature 463:893-898(2010)

PubMed=22675565; DOI=10.1371/journal.pone.0038463; PMCID=PMC3366948
Atak Z.K., De Keersmaecker K., Gianfelici V., Geerdens E., Vandepoel R., Pauwels D., Porcu M., Lahortiga I., Brys V., Dirks W.G., Quentmeier H., Cloos J., Cuppens H., Uyttebroeck A., Vandenberghe P., Cools J., Aerts S.
High accuracy mutation detection in leukemia on a selected panel of cancer genes.
PLoS ONE 7:E38463-E38463(2012)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017; PMCID=PMC4967469
Iorio F., Knijnenburg T.A., Vis D.J., Bignell G.R., Menden M.P., Schubert M., Aben N., Goncalves E., Barthorpe S., Lightfoot H., Cokelaer T., Greninger P., van Dyk E., Chang H., de Silva H., Heyn H., Deng X.-M., Egan R.K., Liu Q.-S., Mironenko T., Mitropoulos X., Richardson L., Wang J.-H., Zhang T.-H., Moran S., Sayols S., Soleimani M., Tamborero D., Lopez-Bigas N., Ross-Macdonald P., Esteller M., Gray N.S., Haber D.A., Stratton M.R., Benes C.H., Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747; PMCID=PMC6445675
Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3; PMCID=PMC6697103
Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C., McDonald E.R. 3rd, Barretina J.G., Gelfand E.T., Bielski C.M., Li H.-X., Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F., Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R., Lu Y.-L., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C., Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A., Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D., Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L., Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A., Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D., Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M., Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R., Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A., Sellers W.R.
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)

PubMed=35354797; DOI=10.1038/s41467-022-29224-5; PMCID=PMC8967900
Leo I.R., Aswad L., Stahl M., Kunold E., Post F., Erkers T., Struyf N., Mermelekas G., Joshi R.N., Gracia-Villacampa E., Ostling P., Kallioniemi O.-P., Pokrovskaja Tamm K., Siavelis I., Lehtio J., Vesterlund M., Jafari R.
Integrative multi-omics and drug response profiling of childhood acute lymphoblastic leukemia cell lines.
Nat. Commun. 13:1691.1-1691.19(2022)

PubMed=35839778; DOI=10.1016/j.ccell.2022.06.010; PMCID=PMC9387775
Goncalves E., Poulos R.C., Cai Z.-X., Barthorpe S., Manda S.S., Lucas N., Beck A., Bucio-Noble D., Dausmann M., Hall C., Hecker M., Koh J., Lightfoot H., Mahboob S., Mali I., Morris J., Richardson L., Seneviratne A.J., Shepherd R., Sykes E., Thomas F., Valentini S., Williams S.G., Wu Y.-X., Xavier D., MacKenzie K.L., Hains P.G., Tully B., Robinson P.J., Zhong Q., Garnett M.J., Reddel R.R.
Pan-cancer proteomic map of 949 human cell lines.
Cancer Cell 40:835-849.e8(2022)

Cross-references
Cell line collections (Providers) DSMZ; ACC-105 - Discontinued
ECACC; 06072602
NCBI_Iran; C180
Cell line databases/resources CLO; CLO_0007067
CLDB; cl35
cancercelllines; CVCL_1326
Cell_Model_Passport; SIDM01007
Cosmic-CLP; 907272
DepMap; ACH-002256
DSMZCellDive; ACC-105
LINCS_LDP; LCL-1015
Biological sample resources BioSample; SAMN03473409
Chemistry resources ChEMBL-Cells; CHEMBL3308305
ChEMBL-Targets; CHEMBL2366305
GDSC; 907272
PharmacoDB; KARPAS45_731_2019
PubChem_Cell_line; CVCL_1326
Encyclopedic resources Wikidata; Q54899501
Gene expression databases ArrayExpress; E-MTAB-783
ArrayExpress; E-MTAB-3610
GEO; GSM1669977
GEO; GSM5137719
Polymorphism and mutation databases Cosmic; 907272
Cosmic; 913413
Cosmic; 1012075
Cosmic; 1151781
Cosmic; 1175130
Cosmic; 1191704
Cosmic; 1281370
Cosmic; 1330505
Cosmic; 1524799
Cosmic; 1664523
Cosmic; 1760525
Cosmic; 2165714
Cosmic; 2391812
Cosmic; 2602917
IARC_TP53; 27173
Proteomic databases PRIDE; PXD023662
PRIDE; PXD030304
Sequence databases EGA; EGAS00001000978
Entry history
Entry creation04-Apr-2012
Last entry update02-May-2024
Version number42