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Cellosaurus HCE-T (CVCL_1272)

[Text version]
Cell line name HCE-T
Synonyms HCET; Human Corneal Epithelial cells-Transformed; HCE; SV40-HCEC
Accession CVCL_1272
Resource Identification Initiative To cite this cell line use: HCE-T (RRID:CVCL_1272)
Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Population: Japanese.
Doubling time: 24.4 hours (PubMed=7534282).
Transformant: Ad-SV40 hybrid virus.
Omics: Deep exome analysis.
Omics: DNA methylation analysis.
Omics: SNP array analysis.
Omics: Transcriptome analysis by microarray.
Omics: Transcriptome analysis by RNAseq.
Derived from site: In situ; Eye, cornea, epithelium; UBERON=UBERON_0001772.
Cell type: Epithelial cell of cornea; CL=CL_0000575.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Sex of cell Female
Age at sampling 49Y
Category Transformed cell line
STR profile Source(s): PubMed=26900324; PubMed=29787296; RCB=RCB2280

Markers:
AmelogeninX
CSF1PO9,12 (PubMed=26900324)
12 (RCB=RCB2280; PubMed=29787296)
D2S133819,20
D3S135815,17
D5S8189,13 (PubMed=26900324; PubMed=29787296)
13 (RCB=RCB2280)
D7S82010,13
D8S117911,13
D13S3178
D16S5399,11
D18S5113 (PubMed=29787296)
13,15 (PubMed=26900324)
D19S43313,14
D21S1127,30
FGA20
Penta D12,15
Penta E15,16
TH017,8
TPOX8,11
vWA18,19

Run an STR similarity search on this cell line
Publications

PubMed=7534282
Araki-Sasaki K., Ohashi Y., Sasabe T., Hayashi K., Watanabe H., Tano Y., Handa H.
An SV40-immortalized human corneal epithelial cell line and its characterization.
Invest. Ophthalmol. Vis. Sci. 36:614-621(1995)

PubMed=20164919; DOI=10.1038/nature08768; PMCID=PMC3145113
Bignell G.R., Greenman C.D., Davies H.R., Butler A.P., Edkins S., Andrews J.M., Buck G., Chen L., Beare D., Latimer C., Widaa S., Hinton J., Fahey C., Fu B.-Y., Swamy S., Dalgliesh G.L., Teh B.T., Deloukas P., Yang F.-T., Campbell P.J., Futreal P.A., Stratton M.R.
Signatures of mutation and selection in the cancer genome.
Nature 463:893-898(2010)

PubMed=26900324; PMCID=PMC4734150
Akin D., Newman J.R.B., McIntyre L.M., Sugrue S.P.
RNA-seq analysis of impact of PNN on gene expression and alternative splicing in corneal epithelial cells.
Mol. Vis. 22:40-60(2016)

PubMed=28315467; DOI=10.1016/j.ejps.2017.03.018
Juretic M., Jurisic Dukovski B., Krtalic I., Reichl S., Cetina-Cizmek B., Filipovic-Grcic J., Lovric J., Pepic I.
HCE-T cell-based permeability model: a well-maintained or a highly variable barrier phenotype?
Eur. J. Pharm. Sci. 104:23-30(2017)

DOI=10.22028/D291-35665
Rubelowski A.-K.
Characterization of the HCE-T cell line (RCB 2280) and its comparison with primary limbal epithelial cell culture and primary corneal epithelial cells.
Thesis MD (2018); Saarland University; Saarbrucken; Germany

PubMed=29787296; DOI=10.1080/02713683.2018.1480043; PMCID=PMC6590053
McDermott A.M., Baidouri H., Woodward A.M., Kam W.R., Liu Y., Chen X.-M., Ziemanski J.F., Vistisen K., Hazlett L.D., Nichols K.K., Argueso P., Sullivan D.A.
Short tandem repeat (STR) profiles of commonly used human ocular surface cell lines.
Curr. Eye Res. 43:1097-1101(2018)

PubMed=31927639; DOI=10.1007/s00417-019-04563-0
Rubelowski A.-K., Latta L., Katiyar P., Stachon T., Kasmann-Kellner B., Seitz B., Szentmary N.
HCE-T cell line lacks cornea-specific differentiation markers compared to primary limbal epithelial cells and differentiated corneal epithelium.
Graefes Arch. Clin. Exp. Ophthalmol. 258:565-575(2020)

Cross-references
Cell line collections (Providers) BCRJ; 0349 - Discontinued
RCB; RCB1384 - Discontinued
RCB; RCB2280
Cell line databases/resources CLO; CLO_0050405
CLO; CLO_0050406
Cell_Model_Passport; SIDM00075
DepMap; ACH-002336
FCS-free; 4-2-4-2-4-3
FCS-free; 4-2-6-1-4-3
LINCS_LDP; LCL-1047
Anatomy/cell type resources BTO; BTO:0006396
Biological sample resources BioSample; SAMN03472134
Chemistry resources ChEMBL-Cells; CHEMBL3308200
ChEMBL-Targets; CHEMBL2366139
PharmacoDB; HCET_516_2019
PubChem_Cell_line; CVCL_1272
Encyclopedic resources Wikidata; Q54881856
Gene expression databases ArrayExpress; E-MTAB-783
ArrayExpress; E-MTAB-3610
GEO; GSE73060
GEO; GSM1669867
Polymorphism and mutation databases Cosmic; 907049
IARC_TP53; 27695
Sequence databases EGA; EGAS00001000978
Entry history
Entry creation04-Apr-2012
Last entry update02-May-2024
Version number29