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Cellosaurus H4 (CVCL_1239)

[Text version]
Cell line name H4
Synonyms H-4
Accession CVCL_1239
Resource Identification Initiative To cite this cell line use: H4 (RRID:CVCL_1239)
Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: COSMIC cell lines project.
Part of: PTEN genetic alteration cell panel (ATCC TCP-1030).
Part of: TCGA-110-CL cell line panel.
Population: Caucasian.
Microsatellite instability: Stable (MSS) (Sanger).
Omics: Deep exome analysis.
Omics: Deep quantitative proteome analysis.
Omics: DNA methylation analysis.
Omics: SNP array analysis.
Omics: Transcriptome analysis by microarray.
Omics: Transcriptome analysis by RNAseq.
Miscellaneous: Originally classified as originating from a neuroglioma, an obsolete classification. This cell line has been reclassified as an astrocytoma.
Derived from site: In situ; Brain; UBERON=UBERON_0000955.
Sequence variations
  • Gene deletion; HGNC; 9588; PTEN; Zygosity=Homozygous (PubMed=10560660).
  • Mutation; HGNC; 5382; IDH1; None_reported; -; Zygosity=- (PubMed=19435942).
  • Mutation; HGNC; 11730; TERT; Simple; c.1-124C>T (c.228C>T) (C228T); Zygosity=Unspecified; Note=In promoter (PubMed=31068700).
  • Mutation; HGNC; 11998; TP53; None_reported; -; Zygosity=- (Cosmic-CLP=907042; DepMap=ACH-000389).
HLA typing Source: PubMed=26589293
Class I
HLA-AA*03:01,30:02
HLA-BB*08:01,18:01
HLA-CC*05:01,07:01
Class II
HLA-DQDQB1*02:02,02:02
HLA-DRDRB1*03:01,03:01
Genome ancestry Source: PubMed=30894373

Origin% genome
African0
Native American1.32
East Asian, North0.97
East Asian, South0
South Asian0
European, North68.05
European, South29.66
Disease Astrocytoma (NCIt: C60781)
Astrocytoma (ORDO: Orphanet_94)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_D601 (H4-APP751)CVCL_U993 (H4-C99)
Sex of cell Male
Age at sampling 37Y
Category Cancer cell line
STR profile Source(s): ATCC=HTB-148; CCRID; CLS=300184; Cosmic-CLP=907042

Markers:
AmelogeninX,Y
CSF1PO10,12
D2S133818,25
D3S135817,18
D5S81810,12
D7S8208,11
D8S11799,12 (ATCC=HTB-148; CCRID)
14 (CLS=300184)
D13S31712
D16S53911,12
D18S5114,16
D19S43312,14
D21S1130,31
FGA19,25
Penta D10,12
Penta E5,12
TH017,9
TPOX8,11
vWA14,18

Run an STR similarity search on this cell line
Web pages https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/h/cell-lines-detail-293.html
Publications

PubMed=4544026; DOI=10.1093/jnci/52.1.71
Arnstein P., Taylor D.O.N., Nelson-Rees W.A., Huebner R.J., Lennette E.H.
Propagation of human tumors in antithymocyte serum-treated mice.
J. Natl. Cancer Inst. 52:71-84(1974)

PubMed=6256643; DOI=10.1038/288724a0
Day R.S. 3rd, Ziolkowski C.H.J., Scudiero D.A., Meyer S.A., Lubiniecki A.S., Girardi A.J., Galloway S.M., Bynum G.D.
Defective repair of alkylated DNA by human tumour and SV40-transformed human cell strains.
Nature 288:724-727(1980)

PubMed=6500159; DOI=10.1159/000163283
Gershwin M.E., Lentz D., Owens R.B.
Relationship between karyotype of tissue culture lines and tumorigenicity in nude mice.
Exp. Cell Biol. 52:361-370(1984)

DOI=10.1007/0-306-46861-1_11
Ali-Osman F.
Brain tumors.
(In book chapter) Human cell culture. Vol. 2. Cancer cell lines part 2; Masters J.R.W., Palsson B.O. (eds.); pp.167-184; Kluwer Academic Publishers; New York; USA (1999)

PubMed=10074188; DOI=10.1128/JVI.73.4.3338-3350.1999; PMCID=PMC104098
Arbour N., Cote G., Lachance C., Tardieu M., Cashman N.R., Talbot P.J.
Acute and persistent infection of human neural cell lines by human coronavirus OC43.
J. Virol. 73:3338-3350(1999)

PubMed=10560660; DOI=10.1097/00005072-199911000-00007
Schmidt E.E., Ichimura K., Goike H.M., Moshref A., Liu L., Collins V.P.
Mutational profile of the PTEN gene in primary human astrocytic tumors and cultivated xenografts.
J. Neuropathol. Exp. Neurol. 58:1170-1183(1999)

PubMed=11414198; DOI=10.1007/s004320000207
Lahm H., Andre S., Hoeflich A., Fischer J.R., Sordat B., Kaltner H., Wolf E., Gabius H.-J.
Comprehensive galectin fingerprinting in a panel of 61 human tumor cell lines by RT-PCR and its implications for diagnostic and therapeutic procedures.
J. Cancer Res. Clin. Oncol. 127:375-386(2001)

PubMed=11508811; DOI=10.1023/A:1010680920087
Krex D., Mohr B., Hauses M., Ehninger G., Schackert H.K., Schackert G.
Identification of uncommon chromosomal aberrations in the neuroglioma cell line H4 by spectral karyotyping.
J. Neurooncol. 52:119-128(2001)

PubMed=19435942; DOI=10.1215/15228517-2009-025; PMCID=PMC2743214
Ichimura K., Pearson D.M., Kocialkowski S., Backlund L.M., Chan R., Jones D.T.W., Collins V.P.
IDH1 mutations are present in the majority of common adult gliomas but rare in primary glioblastomas.
Neuro-oncol. 11:341-347(2009)

PubMed=20164919; DOI=10.1038/nature08768; PMCID=PMC3145113
Bignell G.R., Greenman C.D., Davies H.R., Butler A.P., Edkins S., Andrews J.M., Buck G., Chen L., Beare D., Latimer C., Widaa S., Hinton J., Fahey C., Fu B.-Y., Swamy S., Dalgliesh G.L., Teh B.T., Deloukas P., Yang F.-T., Campbell P.J., Futreal P.A., Stratton M.R.
Signatures of mutation and selection in the cancer genome.
Nature 463:893-898(2010)

PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458; PMCID=PMC2881662
Rothenberg S.M., Mohapatra G., Rivera M.N., Winokur D., Greninger P., Nitta M., Sadow P.M., Sooriyakumar G., Brannigan B.W., Ulman M.J., Perera R.M., Wang R., Tam A., Ma X.-J., Erlander M., Sgroi D.C., Rocco J.W., Lingen M.W., Cohen E.E.W., Louis D.N., Settleman J., Haber D.A.
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
Cancer Res. 70:2158-2164(2010)

PubMed=22460905; DOI=10.1038/nature11003; PMCID=PMC3320027
Barretina J.G., Caponigro G., Stransky N., Venkatesan K., Margolin A.A., Kim S., Wilson C.J., Lehar J., Kryukov G.V., Sonkin D., Reddy A., Liu M., Murray L., Berger M.F., Monahan J.E., Morais P., Meltzer J., Korejwa A., Jane-Valbuena J., Mapa F.A., Thibault J., Bric-Furlong E., Raman P., Shipway A., Engels I.H., Cheng J., Yu G.-Y.K., Yu J.-J., Aspesi P. Jr., de Silva M., Jagtap K., Jones M.D., Wang L., Hatton C., Palescandolo E., Gupta S., Mahan S., Sougnez C., Onofrio R.C., Liefeld T., MacConaill L.E., Winckler W., Reich M., Li N.-X., Mesirov J.P., Gabriel S.B., Getz G., Ardlie K., Chan V., Myer V.E., Weber B.L., Porter J., Warmuth M., Finan P., Harris J.L., Meyerson M.L., Golub T.R., Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Nature 483:603-607(2012)

PubMed=26589293; DOI=10.1186/s13073-015-0240-5; PMCID=PMC4653878
Scholtalbers J., Boegel S., Bukur T., Byl M., Goerges S., Sorn P., Loewer M., Sahin U., Castle J.C.
TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.
Genome Med. 7:118.1-118.7(2015)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017; PMCID=PMC4967469
Iorio F., Knijnenburg T.A., Vis D.J., Bignell G.R., Menden M.P., Schubert M., Aben N., Goncalves E., Barthorpe S., Lightfoot H., Cokelaer T., Greninger P., van Dyk E., Chang H., de Silva H., Heyn H., Deng X.-M., Egan R.K., Liu Q.-S., Mironenko T., Mitropoulos X., Richardson L., Wang J.-H., Zhang T.-H., Moran S., Sayols S., Soleimani M., Tamborero D., Lopez-Bigas N., Ross-Macdonald P., Esteller M., Gray N.S., Haber D.A., Stratton M.R., Benes C.H., Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747; PMCID=PMC6445675
Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3; PMCID=PMC6697103
Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C., McDonald E.R. 3rd, Barretina J.G., Gelfand E.T., Bielski C.M., Li H.-X., Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F., Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R., Lu Y.-L., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C., Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A., Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D., Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L., Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A., Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D., Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M., Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R., Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A., Sellers W.R.
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)

PubMed=31395879; DOI=10.1038/s41467-019-11415-2; PMCID=PMC6687785
Yu K., Chen B., Aran D., Charalel J., Yau C., Wolf D.M., van 't Veer L.J., Butte A.J., Goldstein T., Sirota M.
Comprehensive transcriptomic analysis of cell lines as models of primary tumors across 22 tumor types.
Nat. Commun. 10:3574.1-3574.11(2019)

PubMed=35839778; DOI=10.1016/j.ccell.2022.06.010; PMCID=PMC9387775
Goncalves E., Poulos R.C., Cai Z.-X., Barthorpe S., Manda S.S., Lucas N., Beck A., Bucio-Noble D., Dausmann M., Hall C., Hecker M., Koh J., Lightfoot H., Mahboob S., Mali I., Morris J., Richardson L., Seneviratne A.J., Shepherd R., Sykes E., Thomas F., Valentini S., Williams S.G., Wu Y.-X., Xavier D., MacKenzie K.L., Hains P.G., Tully B., Robinson P.J., Zhong Q., Garnett M.J., Reddel R.R.
Pan-cancer proteomic map of 949 human cell lines.
Cancer Cell 40:835-849.e8(2022)

Cross-references
Cell line collections (Providers) ATCC; HTB-148
BCRJ; 0263
CCTCC; GDC0128
CLS; 300184
IZSLER; BS TCL 102
KCB; KCB 2012027YJ
Cell line databases/resources CLO; CLO_0003599
CLDB; cl5193
cancercelllines; CVCL_1239
CCRID; 1101HUM-PUMC000372
CCRID; 4201HUM-CCTCC00128
Cell_Model_Passport; SIDM00852
Cosmic-CLP; 907042
DepMap; ACH-000389
IGRhCellID; H4
LINCS_LDP; LCL-1401
Lonza; 285
TOKU-E; 1367
Anatomy/cell type resources BTO; BTO:0003704
Biological sample resources BioSample; SAMN03472722
BioSample; SAMN10987915
ENCODE; ENCBS381KGR
ENCODE; ENCBS836LDB
ENCODE; ENCBS902ISQ
CRISP screens repositories BioGRID_ORCS_Cell_line; 430
Chemistry resources ChEMBL-Cells; CHEMBL3308507
ChEMBL-Targets; CHEMBL1075452
GDSC; 907042
PharmacoDB; H4_437_2019
PubChem_Cell_line; CVCL_1239
Encyclopedic resources Wikidata; Q54872126
Experimental variables resources EFO; EFO_0002184
Gene expression databases ArrayExpress; E-MTAB-38
ArrayExpress; E-MTAB-783
ArrayExpress; E-MTAB-2770
ArrayExpress; E-MTAB-3610
GEO; GSM326236
GEO; GSM887031
GEO; GSM888101
GEO; GSM1669834
Polymorphism and mutation databases Cosmic; 687571
Cosmic; 849856
Cosmic; 907042
Cosmic; 1610749
Cosmic; 1746955
Cosmic; 2060805
Cosmic; 2302320
Cosmic; 2367529
IARC_TP53; 21355
LiGeA; CCLE_383
Progenetix; CVCL_1239
Proteomic databases PRIDE; PXD030304
Sequence databases EGA; EGAS00001000978
Entry history
Entry creation04-Apr-2012
Last entry update02-May-2024
Version number42