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Cellosaurus CAL-27 (CVCL_1107)

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Cell line name CAL-27
Synonyms Cal-27; CAL 27; Cal 27; CAL27; Cal27; CAL-SCC-27; Centre Antoine Lacassagne-27
Accession CVCL_1107
Resource Identification Initiative To cite this cell line use: CAL-27 (RRID:CVCL_1107)
Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: COSMIC cell lines project.
Part of: KuDOS 95 cell line panel.
Part of: MD Anderson Cell Lines Project.
Part of: OPC-22 oral and pharyngeal cancer cell line panel.
Population: Caucasian.
Doubling time: 45 hours (PubMed=2145926); ~35 hours (ATCC=CRL-2095); ~20-40 hours (DSMZ=ACC-446).
Microsatellite instability: Stable (MSS) (Sanger).
Omics: Array-based CGH.
Omics: CRISPR phenotypic screen.
Omics: Deep exome analysis.
Omics: Deep quantitative proteome analysis.
Omics: DNA methylation analysis.
Omics: Protein expression by reverse-phase protein arrays.
Omics: SNP array analysis.
Omics: Transcriptome analysis by microarray.
Omics: Transcriptome analysis by RNAseq.
Derived from site: In situ; Oral cavity, tongue; UBERON=UBERON_0001723.
Sequence variations
  • Mutation; HGNC; 11730; TERT; Simple; c.1-124C>T (c.228C>T) (C228T); Zygosity=Unspecified; Note=In promoter (PubMed=31068700).
  • Mutation; HGNC; 11998; TP53; Simple; p.His193Leu (c.578A>T); ClinVar=VCV000185822; Zygosity=Unspecified (PubMed=25275298).
HLA typing Source: PubMed=26589293
Class I
HLA-AA*26:08,26:08
HLA-BB*44:03,51:01
HLA-CC*07:02,16:01
Class II
HLA-DQDQA1*01:02,02:01
DQB1*05:01,05:01
HLA-DRDRB1*01:03,07:01
Genome ancestry Source: PubMed=30894373

Origin% genome
African2.22
Native American0
East Asian, North2.6
East Asian, South0
South Asian0
European, North54.66
European, South40.51
Disease Tongue adenosquamous carcinoma (NCIt: C173807)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_C0RK (CAL-27 2B1)CVCL_C0RL (CAL-27 2B3)CVCL_GY99 (CAL-27 TPFR)
CVCL_EI26 (ER-Cal-27)
Sex of cell Male
Age at sampling 56Y
Category Cancer cell line
STR profile Source(s): ATCC=CRL-2095; CCRID; Cosmic-CLP=910916; DSMZ=ACC-446; PubMed=19631575; PubMed=21868764; PubMed=25275298; PubMed=25877200; PubMed=36912284; Technion Genomics Center

Markers:
AmelogeninX
CSF1PO10,12
D1S165613,16
D2S44110,11.3
D2S133823,24
D3S135816
D5S81811,12
D6S104312
D7S82010
D8S117913,15
D10S124812,16
D12S39118.3,20
D13S31710,11
D16S53911,12
D18S5113
D19S43314,15.2 (ATCC=CRL-2095; CCRID; Technion Genomics Center)
14,16 (DSMZ=ACC-446)
D21S1128,29
D22S104513,16
FGA21,25 (CCRID)
25 (ATCC=CRL-2095; DSMZ=ACC-446; PubMed=19631575; PubMed=25275298; PubMed=25877200; Technion Genomics Center)
Penta D9,10
Penta E7
TH016,9.3
TPOX8
vWA14,17

Run an STR similarity search on this cell line
Web pages https://tcpaportal.org/mclp/
Publications

PubMed=2440566
Gioanni J., Samson M., Zanghellini E., Mazeau C., Ettore F., Demard F., Chauvel P., Duplay H., Schneider M., Laurent J.-C., Lalanne C.-M.
Characterization of a new surface epitope specific for human epithelial cells defined by a monoclonal antibody and application to tumor diagnosis.
Cancer Res. 47:4417-4424(1987)

PubMed=3181269; DOI=10.1016/0277-5379(88)90335-5
Gioanni J., Fischel J.-L., Lambert J.-C., Demard F., Mazeau C., Zanghellini E., Ettore F., Formento P., Chauvel P., Lalanne C.-M., Courdi A.
Two new human tumor cell lines derived from squamous cell carcinomas of the tongue: establishment, characterization and response to cytotoxic treatment.
Eur. J. Cancer Clin. Oncol. 24:1445-1455(1988)

PubMed=2145926; DOI=10.1016/0277-5379(90)90186-W
Olivier S., Formento P., Fischel J.-L., Etienne-Grimaldi M.-C., Milano G.
Epidermal growth factor receptor expression and suramin cytotoxicity in vitro.
Eur. J. Cancer 26:867-871(1990)

PubMed=19631575; DOI=10.1016/j.oraloncology.2009.06.001
Jiang L., Ji N., Zhou Y., Li J., Liu X.-T., Wang Z., Chen Q.-M., Zeng X.
CAL 27 is an oral adenosquamous carcinoma cell line.
Oral Oncol. 45:e204-e207(2009)

PubMed=20014447; DOI=10.1002/hed.21311; PMCID=PMC2930055
Tsui I.F.L., Garnis C.
Integrative molecular characterization of head and neck cancer cell model genomes.
Head Neck 32:1143-1160(2010)

PubMed=20164919; DOI=10.1038/nature08768; PMCID=PMC3145113
Bignell G.R., Greenman C.D., Davies H.R., Butler A.P., Edkins S., Andrews J.M., Buck G., Chen L., Beare D., Latimer C., Widaa S., Hinton J., Fahey C., Fu B.-Y., Swamy S., Dalgliesh G.L., Teh B.T., Deloukas P., Yang F.-T., Campbell P.J., Futreal P.A., Stratton M.R.
Signatures of mutation and selection in the cancer genome.
Nature 463:893-898(2010)

PubMed=21868764; DOI=10.1158/1078-0432.CCR-11-0690; PMCID=PMC3229662
Zhao M., Sano D., Pickering C.R., Jasser S.A., Henderson Y.C., Clayman G.L., Sturgis E.M., Ow T.J., Lotan R., Carey T.E., Sacks P.G., Grandis J.R., Sidransky D., Heldin N.-E., Myers J.N.
Assembly and initial characterization of a panel of 85 genomically validated cell lines from diverse head and neck tumor sites.
Clin. Cancer Res. 17:7248-7264(2011)

PubMed=22460905; DOI=10.1038/nature11003; PMCID=PMC3320027
Barretina J.G., Caponigro G., Stransky N., Venkatesan K., Margolin A.A., Kim S., Wilson C.J., Lehar J., Kryukov G.V., Sonkin D., Reddy A., Liu M., Murray L., Berger M.F., Monahan J.E., Morais P., Meltzer J., Korejwa A., Jane-Valbuena J., Mapa F.A., Thibault J., Bric-Furlong E., Raman P., Shipway A., Engels I.H., Cheng J., Yu G.-Y.K., Yu J.-J., Aspesi P. Jr., de Silva M., Jagtap K., Jones M.D., Wang L., Hatton C., Palescandolo E., Gupta S., Mahan S., Sougnez C., Onofrio R.C., Liefeld T., MacConaill L.E., Winckler W., Reich M., Li N.-X., Mesirov J.P., Gabriel S.B., Getz G., Ardlie K., Chan V., Myer V.E., Weber B.L., Porter J., Warmuth M., Finan P., Harris J.L., Meyerson M.L., Golub T.R., Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Nature 483:603-607(2012)

PubMed=24201445; DOI=10.1101/gr.164806.113; PMCID=PMC3912410
Akagi K., Li J.-F., Broutian T.R., Padilla-Nash H.M., Xiao W.-H., Jiang B., Rocco J.W., Teknos T.N., Kumar B., Wangsa D., He D., Ried T., Symer D.E., Gillison M.L.
Genome-wide analysis of HPV integration in human cancers reveals recurrent, focal genomic instability.
Genome Res. 24:185-199(2014)

PubMed=25275298; DOI=10.18632/oncotarget.2417; PMCID=PMC4253406
Martin D., Abba M.C., Molinolo A.A., Vitale-Cross L., Wang Z.-Y., Zaida M., Delic N.C., Samuels Y., Lyons J.G., Gutkind J.S.
The head and neck cancer cell oncogenome: a platform for the development of precision molecular therapies.
Oncotarget 5:8906-8923(2014)

PubMed=25485619; DOI=10.1038/nbt.3080
Klijn C., Durinck S., Stawiski E.W., Haverty P.M., Jiang Z.-S., Liu H.-B., Degenhardt J., Mayba O., Gnad F., Liu J.-F., Pau G., Reeder J., Cao Y., Mukhyala K., Selvaraj S.K., Yu M.-M., Zynda G.J., Brauer M.J., Wu T.D., Gentleman R.C., Manning G., Yauch R.L., Bourgon R., Stokoe D., Modrusan Z., Neve R.M., de Sauvage F.J., Settleman J., Seshagiri S., Zhang Z.-M.
A comprehensive transcriptional portrait of human cancer cell lines.
Nat. Biotechnol. 33:306-312(2015)

PubMed=25877200; DOI=10.1038/nature14397
Yu M., Selvaraj S.K., Liang-Chu M.M.Y., Aghajani S., Busse M., Yuan J., Lee G., Peale F.V., Klijn C., Bourgon R., Kaminker J.S., Neve R.M.
A resource for cell line authentication, annotation and quality control.
Nature 520:307-311(2015)

PubMed=25888065; DOI=10.1016/j.molonc.2015.03.008; PMCID=PMC4523436
Stanam A., Love-Homan L., Joseph T.S., Espinosa-Cotton M., Simons A.L.
Upregulated interleukin-6 expression contributes to erlotinib resistance in head and neck squamous cell carcinoma.
Mol. Oncol. 9:1371-1383(2015)

PubMed=26589293; DOI=10.1186/s13073-015-0240-5; PMCID=PMC4653878
Scholtalbers J., Boegel S., Bukur T., Byl M., Goerges S., Sorn P., Loewer M., Sahin U., Castle J.C.
TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.
Genome Med. 7:118.1-118.7(2015)

PubMed=27108184; DOI=10.1016/j.bbamcr.2016.04.019
Stojanovic N., Brozovic A., Majhen D., Bosnar M.H., Fritz G., Osmak M., Ambriovic-Ristov A.
Integrin alphavbeta3 expression in tongue squamous carcinoma cells Cal27 confers anticancer drug resistance through loss of pSrc(Y418).
Biochim. Biophys. Acta 1863:1969-1978(2016)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017; PMCID=PMC4967469
Iorio F., Knijnenburg T.A., Vis D.J., Bignell G.R., Menden M.P., Schubert M., Aben N., Goncalves E., Barthorpe S., Lightfoot H., Cokelaer T., Greninger P., van Dyk E., Chang H., de Silva H., Heyn H., Deng X.-M., Egan R.K., Liu Q.-S., Mironenko T., Mitropoulos X., Richardson L., Wang J.-H., Zhang T.-H., Moran S., Sayols S., Soleimani M., Tamborero D., Lopez-Bigas N., Ross-Macdonald P., Esteller M., Gray N.S., Haber D.A., Stratton M.R., Benes C.H., Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

PubMed=28196595; DOI=10.1016/j.ccell.2017.01.005; PMCID=PMC5501076
Li J., Zhao W., Akbani R., Liu W.-B., Ju Z.-L., Ling S.-Y., Vellano C.P., Roebuck P., Yu Q.-H., Eterovic A.K., Byers L.A., Davies M.A., Deng W.-L., Gopal Y.N.V., Chen G., von Euw E.M., Slamon D.J., Conklin D., Heymach J.V., Gazdar A.F., Minna J.D., Myers J.N., Lu Y.-L., Mills G.B., Liang H.
Characterization of human cancer cell lines by reverse-phase protein arrays.
Cancer Cell 31:225-239(2017)

PubMed=28476783; DOI=10.21873/anticanres.11555
Hagemann J., Jacobi C., Hahn M., Schmid V., Welz C., Schwenk-Zieger S., Stauber R.H., Baumeister P., Becker S.
Spheroid-based 3D cell cultures enable personalized therapy testing and drug discovery in head and neck cancer.
Anticancer Res. 37:2201-2210(2017)

PubMed=29156801; DOI=10.18632/oncotarget.21174; PMCID=PMC5689691
Kalu N.N., Mazumdar T., Peng S.-H., Shen L., Sambandam V., Rao X.-Y., Xi Y.-X., Li L.-R., Qi Y., Gleber-Netto F.O., Patel A., Wang J., Frederick M.J., Myers J.N., Pickering C.R., Johnson F.M.
Genomic characterization of human papillomavirus-positive and -negative human squamous cell cancer cell lines.
Oncotarget 8:86369-86383(2017)

PubMed=29770536; DOI=10.1111/cas.13640; PMCID=PMC6172064
Yang J., Li L., Xi Y., Sun R.-M., Wang H., Ren Y.-X., Zhao L.-F., Wang X.-L., Li X.-Y.
Combination of IFITM1 knockdown and radiotherapy inhibits the growth of oral cancer.
Cancer Sci. 109:3115-3128(2018)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747; PMCID=PMC6445675
Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=30971826; DOI=10.1038/s41586-019-1103-9
Behan F.M., Iorio F., Picco G., Goncalves E., Beaver C.M., Migliardi G., Santos R., Rao Y., Sassi F., Pinnelli M., Ansari R., Harper S., Jackson D.A., McRae R., Pooley R., Wilkinson P., van der Meer D.J., Dow D., Buser-Doepner C.A., Bertotti A., Trusolino L., Stronach E.A., Saez-Rodriguez J., Yusa K., Garnett M.J.
Prioritization of cancer therapeutic targets using CRISPR-Cas9 screens.
Nature 568:511-516(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3; PMCID=PMC6697103
Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C., McDonald E.R. 3rd, Barretina J.G., Gelfand E.T., Bielski C.M., Li H.-X., Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F., Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R., Lu Y.-L., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C., Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A., Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D., Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L., Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A., Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D., Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M., Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R., Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A., Sellers W.R.
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)

PubMed=31978347; DOI=10.1016/j.cell.2019.12.023; PMCID=PMC7339254
Nusinow D.P., Szpyt J., Ghandi M., Rose C.M., McDonald E.R. 3rd, Kalocsay M., Jane-Valbuena J., Gelfand E.T., Schweppe D.K., Jedrychowski M.P., Golji J., Porter D.A., Rejtar T., Wang Y.K., Kryukov G.V., Stegmeier F., Erickson B.K., Garraway L.A., Sellers W.R., Gygi S.P.
Quantitative proteomics of the Cancer Cell Line Encyclopedia.
Cell 180:387-402.e16(2020)

PubMed=34977030; DOI=10.3389/fcell.2021.786758; PMCID=PMC8716755
Tadijan A., Humphries J.D., Samarzija I., Stojanovic N., Zha J.-Z., Culjak K., Tomic M., Paradzik M., Nestic D., Kang H., Humphries M.J., Ambriovic-Ristov A.
The tongue squamous carcinoma cell line Cal27 primarily employs integrin alpha6beta4-containing type II hemidesmosomes for adhesion which contribute to anticancer drug sensitivity.
Front. Cell Dev. Biol. 9:786758.1-786758.17(2021)

PubMed=35839778; DOI=10.1016/j.ccell.2022.06.010; PMCID=PMC9387775
Goncalves E., Poulos R.C., Cai Z.-X., Barthorpe S., Manda S.S., Lucas N., Beck A., Bucio-Noble D., Dausmann M., Hall C., Hecker M., Koh J., Lightfoot H., Mahboob S., Mali I., Morris J., Richardson L., Seneviratne A.J., Shepherd R., Sykes E., Thomas F., Valentini S., Williams S.G., Wu Y.-X., Xavier D., MacKenzie K.L., Hains P.G., Tully B., Robinson P.J., Zhong Q., Garnett M.J., Reddel R.R.
Pan-cancer proteomic map of 949 human cell lines.
Cancer Cell 40:835-849.e8(2022)

PubMed=36912284; DOI=10.1002/ijc.34506
Nguyen H.T., Tang W.-L., Webster A.L.H., Whiteaker J.R., Chandler C.M., Errazquin Ciudad R., Roohollahi K., Fritzke M., Hoskins E.E., Jonlin E.C., Wakefield L., Sullivan L.B., Chen E.Y., Dorsman J.C., Brakenhoff R.H., Paulovich A.G., Grompe M., Garcia-Escudero R., Wells S.I., Smogorzewska A., Monnat R.J. Jr.
Fanconi anemia-isogenic head and neck cancer cell line pairs: a basic and translational science resource.
Int. J. Cancer 153:183-196(2023)

Cross-references
Cell line collections (Providers) ATCC; CRL-2095
BCRJ; 0326
CCTCC; GDC0248
CLS; 305029
DSMZ; ACC-446
Cell line databases/resources CLO; CLO_0002178
CLO; CLO_0002182
cancercelllines; CVCL_1107
CCRID; 1101HUM-PUMC000338
CCRID; 4201HUM-CCTCC00248
Cell_Model_Passport; SIDM00937
Cosmic-CLP; 910916
DepMap; ACH-000832
DSMZCellDive; ACC-446
IGRhCellID; CAL27%20GSE9585
LINCS_LDP; LCL-1215
Anatomy/cell type resources BTO; BTO:0000921
Biological sample resources BioSample; SAMN03471886
BioSample; SAMN10987905
CRISP screens repositories BioGRID_ORCS_Cell_line; 336
Chemistry resources ChEMBL-Cells; CHEMBL3308098
ChEMBL-Targets; CHEMBL1075408
GDSC; 910916
PharmacoDB; CAL27_168_2019
PubChem_Cell_line; CVCL_1107
Encyclopedic resources Wikidata; Q54808394
Experimental variables resources EFO; EFO_0006366
Gene expression databases ArrayExpress; E-MTAB-783
ArrayExpress; E-MTAB-2706
ArrayExpress; E-MTAB-2770
ArrayExpress; E-MTAB-3610
GEO; GSM422868
GEO; GSM423671
GEO; GSM886907
GEO; GSM887972
GEO; GSM1374427
GEO; GSM1374428
GEO; GSM1519074
GEO; GSM1519075
GEO; GSM1519076
GEO; GSM1669648
Polymorphism and mutation databases Cosmic; 686972
Cosmic; 910916
Cosmic; 1006469
Cosmic; 1017793
Cosmic; 1041674
Cosmic; 1339913
Cosmic; 1466800
Cosmic; 1530734
Cosmic; 1752762
Cosmic; 2043896
Cosmic; 2131842
Cosmic; 2296292
IARC_TP53; 17482
LiGeA; CCLE_698
Progenetix; CVCL_1107
Proteomic databases PRIDE; PXD002132
PRIDE; PXD028810
PRIDE; PXD029420
PRIDE; PXD030304
Sequence databases EGA; EGAS00001000610
EGA; EGAS00001000978
Entry history
Entry creation04-Apr-2012
Last entry update10-Sep-2024
Version number47