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Cellosaurus MEG-01 (CVCL_0425)

[Text version]
Cell line name MEG-01
Synonyms Meg-01; MEG01; Meg01
Accession CVCL_0425
Resource Identification Initiative To cite this cell line use: MEG-01 (RRID:CVCL_0425)
Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: COSMIC cell lines project.
Population: Japanese.
Characteristics: Can be induced to differentiate into megakaryocytes after application of 12-O-tetradecanoyl-13-phorbol acetate (TPA) or valproic acid (VPA) (PubMed=1742484; PubMed=30332548).
Doubling time: 36-48 hours (PubMed=2998511); ~35 hours (DSMZ=ACC-364).
Microsatellite instability: Stable (MSS) (Sanger).
Omics: Deep exome analysis.
Omics: Deep quantitative proteome analysis.
Omics: DNA methylation analysis.
Omics: SNP array analysis.
Omics: Transcriptome analysis by microarray.
Omics: Transcriptome analysis by RNAseq.
Misspelling: Mego-1; Note=Occasionally.
Derived from site: In situ; Bone marrow; UBERON=UBERON_0002371.
Sequence variations
  • Gene fusion; HGNC; 76; ABL1 + HGNC; 1014; BCR; Name(s)=BCR-ABL1, BCR-ABL; Note=BCR exon 13 fused to ABL1 exon 2 (b2a2 transcript) (PubMed=10071072; PubMed=10576511; PubMed=15843827; PubMed=20809971; DepMap=ACH-000072).
  • Mutation; HGNC; 18318; ASXL1; Simple; p.Gly646Trpfs*12 (c.1934dupG); ClinVar=VCV000426927; Zygosity=Heterozygous (PubMed=26700326; DepMap=ACH-000072).
  • Mutation; HGNC; 11998; TP53; Simple; p.His233del (c.697_699delCAC); Zygosity=Heterozygous (Cosmic-CLP=908126; DepMap=ACH-000072).
Genome ancestry Source: PubMed=30894373

Origin% genome
African1.14
Native American0
East Asian, North79.13
East Asian, South17.4
South Asian0
European, North0
European, South2.33
Disease Blast phase chronic myelogenous leukemia, BCR-ABL1 positive (NCIt: C9110)
Chronic myeloid leukemia (ORDO: Orphanet_521)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_E2Q6 (HyCyte MEG-01 hJAK2_p.V617F_c.1849G>T)CVCL_3022 (MEG-01s)CVCL_VG38 (SPYMEG)
Sex of cell Male
Age at sampling 55Y
Category Cancer cell line
STR profile Source(s): ATCC=CRL-2021; Cosmic-CLP=908126; DSMZ=ACC-364; ECACC=94012401; JCRB=IFO50151

Markers:
AmelogeninX,Y
CSF1PO10
D2S133819
D3S135815
D5S81813
D7S82011
D8S117914,15
D13S3178
D16S5399
D18S5118,22
D19S43314,16
D21S1129
FGA26
Penta D11,13
Penta E15
TH017
TPOX8,11
vWA16

Run an STR similarity search on this cell line
Publications

PubMed=2998511; DOI=10.1182/blood.V66.6.1384.1384
Ogura M., Morishima Y., Ohno R., Kato Y., Hirabayashi N., Nagura H., Saito H.
Establishment of a novel human megakaryoblastic leukemia cell line, MEG-01, with positive Philadelphia chromosome.
Blood 66:1384-1392(1985)

PubMed=3332852; DOI=10.1016/S0950-3536(87)80037-9
Keating A.
Ph positive CML cell lines.
Baillieres Clin. Haematol. 1:1021-1029(1987)

PubMed=1742484; DOI=10.1182/blood.V78.12.3168.3168
Murate T., Hotta T., Tsushita K., Suzuki M., Yoshida T., Saga S., Saito H., Yoshida S.
Aphidicolin, an inhibitor of DNA replication, blocks the TPA-induced differentiation of a human megakaryoblastic cell line, MEG-O1.
Blood 78:3168-3177(1991)

PubMed=9738977; DOI=10.1111/j.1349-7006.1998.tb03275.x; PMCID=PMC5921886
Takizawa J., Suzuki R., Kuroda H., Utsunomiya A., Kagami Y., Joh T., Aizawa Y., Ueda R., Seto M.
Expression of the TCL1 gene at 14q32 in B-cell malignancies but not in adult T-cell leukemia.
Jpn. J. Cancer Res. 89:712-718(1998)

PubMed=10071072; DOI=10.1016/S0145-2126(98)00171-4
Drexler H.G., MacLeod R.A.F., Uphoff C.C.
Leukemia cell lines: in vitro models for the study of Philadelphia chromosome-positive leukemia.
Leuk. Res. 23:207-215(1999)

PubMed=10576511; DOI=10.1016/S0145-2126(99)00131-9
Uphoff C.C., Habig S., Fombonne S., Matsuo Y., Drexler H.G.
ABL-BCR expression in BCR-ABL-positive human leukemia cell lines.
Leuk. Res. 23:1055-1060(1999)

DOI=10.1016/B978-0-12-221970-2.50457-5
Drexler H.G.
The leukemia-lymphoma cell line factsbook.
(In book) ISBN 9780122219702; pp.1-733; Academic Press; London; United Kingdom (2001)

PubMed=15843827; DOI=10.1038/sj.leu.2403749
Andersson A., Eden P., Lindgren D., Nilsson J., Lassen C., Heldrup J., Fontes M., Borg A., Mitelman F., Johansson B., Hoglund M., Fioretos T.
Gene expression profiling of leukemic cell lines reveals conserved molecular signatures among subtypes with specific genetic aberrations.
Leukemia 19:1042-1050(2005)

PubMed=20164919; DOI=10.1038/nature08768; PMCID=PMC3145113
Bignell G.R., Greenman C.D., Davies H.R., Butler A.P., Edkins S., Andrews J.M., Buck G., Chen L., Beare D., Latimer C., Widaa S., Hinton J., Fahey C., Fu B.-Y., Swamy S., Dalgliesh G.L., Teh B.T., Deloukas P., Yang F.-T., Campbell P.J., Futreal P.A., Stratton M.R.
Signatures of mutation and selection in the cancer genome.
Nature 463:893-898(2010)

PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458; PMCID=PMC2881662
Rothenberg S.M., Mohapatra G., Rivera M.N., Winokur D., Greninger P., Nitta M., Sadow P.M., Sooriyakumar G., Brannigan B.W., Ulman M.J., Perera R.M., Wang R., Tam A., Ma X.-J., Erlander M., Sgroi D.C., Rocco J.W., Lingen M.W., Cohen E.E.W., Louis D.N., Settleman J., Haber D.A.
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
Cancer Res. 70:2158-2164(2010)

PubMed=20809971; DOI=10.1186/1755-8166-3-15; PMCID=PMC2944125
Virgili A., Nacheva E.
Genomic amplification of BCR/ABL1 and a region downstream of ABL1 in chronic myeloid leukaemia: a FISH mapping study of CML patients and cell lines.
Mol. Cytogenet. 3:15.1-15.12(2010)

PubMed=22460905; DOI=10.1038/nature11003; PMCID=PMC3320027
Barretina J.G., Caponigro G., Stransky N., Venkatesan K., Margolin A.A., Kim S., Wilson C.J., Lehar J., Kryukov G.V., Sonkin D., Reddy A., Liu M., Murray L., Berger M.F., Monahan J.E., Morais P., Meltzer J., Korejwa A., Jane-Valbuena J., Mapa F.A., Thibault J., Bric-Furlong E., Raman P., Shipway A., Engels I.H., Cheng J., Yu G.-Y.K., Yu J.-J., Aspesi P. Jr., de Silva M., Jagtap K., Jones M.D., Wang L., Hatton C., Palescandolo E., Gupta S., Mahan S., Sougnez C., Onofrio R.C., Liefeld T., MacConaill L.E., Winckler W., Reich M., Li N.-X., Mesirov J.P., Gabriel S.B., Getz G., Ardlie K., Chan V., Myer V.E., Weber B.L., Porter J., Warmuth M., Finan P., Harris J.L., Meyerson M.L., Golub T.R., Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Nature 483:603-607(2012)

PubMed=26700326; DOI=10.1016/j.exphem.2015.11.011
Inoue D., Matsumoto M., Nagase R., Saika M., Fujino T., Nakayama K.I., Kitamura T.
Truncation mutants of ASXL1 observed in myeloid malignancies are expressed at detectable protein levels.
Exp. Hematol. 44:172-176.e1(2016)

PubMed=27277069; DOI=10.1160/TH15-11-0891; PMCID=PMC5080539
Wright J.R., Amisten S., Goodall A.H., Mahaut-Smith M.P.
Transcriptomic analysis of the ion channelome of human platelets and megakaryocytic cell lines.
Thromb. Haemost. 116:272-284(2016)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017; PMCID=PMC4967469
Iorio F., Knijnenburg T.A., Vis D.J., Bignell G.R., Menden M.P., Schubert M., Aben N., Goncalves E., Barthorpe S., Lightfoot H., Cokelaer T., Greninger P., van Dyk E., Chang H., de Silva H., Heyn H., Deng X.-M., Egan R.K., Liu Q.-S., Mironenko T., Mitropoulos X., Richardson L., Wang J.-H., Zhang T.-H., Moran S., Sayols S., Soleimani M., Tamborero D., Lopez-Bigas N., Ross-Macdonald P., Esteller M., Gray N.S., Haber D.A., Stratton M.R., Benes C.H., Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

PubMed=30285677; DOI=10.1186/s12885-018-4840-5; PMCID=PMC6167786
Tan K.-T., Ding L.-W., Sun Q.-Y., Lao Z.-T., Chien W., Ren X., Xiao J.-F., Loh X.-Y., Xu L., Lill M., Mayakonda A., Lin D.-C., Yang H.H., Koeffler H.P.
Profiling the B/T cell receptor repertoire of lymphocyte derived cell lines.
BMC Cancer 18:940.1-940.13(2018)

PubMed=30332548; DOI=10.1080/09537104.2018.1528344
Dhenge A., Kuhikar R., Kale V., Limaye L.S.
Regulation of differentiation of MEG01 to megakaryocytes and platelet-like particles by valproic acid through Notch3 mediated actin polymerization.
Platelets 30:780-795(2019)

PubMed=30629668; DOI=10.1371/journal.pone.0210404; PMCID=PMC6328144
Uphoff C.C., Pommerenke C., Denkmann S.A., Drexler H.G.
Screening human cell lines for viral infections applying RNA-Seq data analysis.
PLoS ONE 14:E0210404-E0210404(2019)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747; PMCID=PMC6445675
Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3; PMCID=PMC6697103
Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C., McDonald E.R. 3rd, Barretina J.G., Gelfand E.T., Bielski C.M., Li H.-X., Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F., Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R., Lu Y.-L., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C., Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A., Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D., Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L., Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A., Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D., Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M., Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R., Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A., Sellers W.R.
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)

PubMed=32180119; DOI=10.1007/s12185-020-02853-6
Saito H., Hayakawa M., Kamoshita N., Yasumoto A., Suzuki-Inoue K., Yatomi Y., Ohmori T.
Establishment of a megakaryoblastic cell line for conventional assessment of platelet calcium signaling.
Int. J. Hematol. 111:786-794(2020)

PubMed=35839778; DOI=10.1016/j.ccell.2022.06.010; PMCID=PMC9387775
Goncalves E., Poulos R.C., Cai Z.-X., Barthorpe S., Manda S.S., Lucas N., Beck A., Bucio-Noble D., Dausmann M., Hall C., Hecker M., Koh J., Lightfoot H., Mahboob S., Mali I., Morris J., Richardson L., Seneviratne A.J., Shepherd R., Sykes E., Thomas F., Valentini S., Williams S.G., Wu Y.-X., Xavier D., MacKenzie K.L., Hains P.G., Tully B., Robinson P.J., Zhong Q., Garnett M.J., Reddel R.R.
Pan-cancer proteomic map of 949 human cell lines.
Cancer Cell 40:835-849.e8(2022)

Cross-references
Cell line collections (Providers) ATCC; CRL-2021
BCRC; 60238
BCRJ; 0262
DSMZ; ACC-364
ECACC; 94012401
ICLC; HTL96017
JCRB; IFO50151
Cell line databases/resources CLO; CLO_0007668
MCCL; MCC:0000322
CLDB; cl3432
CLDB; cl3433
cancercelllines; CVCL_0425
CCRID; 1101HUM-PUMC000521
CCRID; 3101HUMTCHu197
Cell_Model_Passport; SIDM00526
Cosmic-CLP; 908126
DepMap; ACH-000072
DSMZCellDive; ACC-364
IGRhCellID; MEG01
LINCS_LDP; LCL-1110
Lonza; 959
Anatomy/cell type resources BTO; BTO:0002581
Biological sample resources BioSample; SAMN01821577
BioSample; SAMN01821648
BioSample; SAMN01821734
BioSample; SAMN03471278
BioSample; SAMN10988184
Chemistry resources ChEMBL-Cells; CHEMBL3308539
ChEMBL-Targets; CHEMBL2366084
GDSC; 908126
PharmacoDB; MEG01_913_2019
PubChem_Cell_line; CVCL_0425
Encyclopedic resources Wikidata; Q54905035
Experimental variables resources EFO; EFO_0002232
Gene expression databases ArrayExpress; E-MTAB-38
ArrayExpress; E-MTAB-783
ArrayExpress; E-MTAB-2770
ArrayExpress; E-MTAB-3610
GEO; GSM482498
GEO; GSM887307
GEO; GSM888383
GEO; GSM1374669
GEO; GSM1670093
Polymorphism and mutation databases Cosmic; 787503
Cosmic; 908126
Cosmic; 924042
Cosmic; 1026578
Cosmic; 1070702
Cosmic; 1078723
Cosmic; 1516634
Cosmic; 1523830
IARC_TP53; 21491
LiGeA; CCLE_381
Progenetix; CVCL_0425
Proteomic databases PRIDE; PXD030304
Sequence databases EGA; EGAS00001000978
Entry history
Entry creation04-Apr-2012
Last entry update02-May-2024
Version number43